This readme.txt file was generated on December 27, 2020 by Joshua Harrison

GENERAL INFORMATION

1. Title of Dataset: Sequence data from one and two-step library preparation procedures

2. Author Information
	A. Principal Investigator Contact Information
		Name: Joshua G. Harrison
		Institution: University of Wyoming
		Email: Joshua.grant.harrison@gmail.com

3. Date of data collection: 2019--2020

Recommended citation for this dataset: A paper referencing this DOI is forthcoming. Search for this DOI in Google Scholar or other similar search service and cite the associated paper by Joshua G. Harrison, Gregory D. Randolph, and C. Alex Buerkle.


DATA & FILE OVERVIEW

1. File List and metadata:

This tarball contains two directories. The first directory 'coligoSequences' contains data from a library containing coligos and internal standards only. This library was created using our two-step PCR procedure. 

The second directory, called 'exampleOnestep' has sequence data generated from a library made using the one-step PCR procedure we describe in our manuscript.

The file list is as follows:
microbiomeMethodsReview/coligoSequences/
microbiomeMethodsReview/coligoSequences/parsed_iSeq10_S1_L001_R1_001.fastq
microbiomeMethodsReview/coligoSequences/iSeq10BarcodePairsAlteration.csv
microbiomeMethodsReview/coligoSequences/parsed_iSeq10_S1_L001_R2_001.fastq

microbiomeMethodsReview/exampleOnestep/
microbiomeMethodsReview/exampleOnestep/parsed_4AH1-1STEP_S1_L001_R1_001.fastq
microbiomeMethodsReview/exampleOnestep/NS4_4AH1_Demux.csv
microbiomeMethodsReview/exampleOnestep/parsed_4AH1-1STEP_S1_L001_R2_001.fastq

Files prefixed with 'parsed' are demultiplexed sequence data. Csv files contain demultiplexing keys.


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
See forthcoming paper for details.